Application
Western blot
Sample
Mouse Recombinant protein (293 cells transfected with a V5-tagged construct)
Loading amount
2000 cells
Specification
293 cells transfected with a V5-tagged construct
Gel Running Conditions
Reduced Denaturing (10% AA gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: EnVision Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/200
Detection
Detection method
ECL advance
Exposure
10 second(s)
Bands
Specific: 60 kDa
Negative control
Mock-transfected 293 cells.
Additional data
Additional Notes
Degraded protein was observed in the culture supernatant.
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提交于 Nov 19 2012