Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Goat Tissue sections (Ureter)
Specification
Ureter
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: anti IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Urothelium is negative.
Lower dilution factors only increase non-specific staining.
Abcam response
Thank you for your feedback. ab78196 has not been previously tested in goat tissue and your data suggests that ab78196 may not be suitable to detect Uroplakin III in goat tissue under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Jun 02 2014