Anti-UXT抗体(ab77483)
Key features and details
- Goat polyclonal to UXT
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-UXT抗体
参阅全部 UXT 一抗 -
描述
山羊多克隆抗体to UXT -
宿主
Goat -
特异性
ab77483 is expected to recognize both reported isoforms (NP_004173.1; NP_705582.1). -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Horse, Cow, Dog, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide:
C-DHRDKVYEQLAKY
, from an internal region of Human UXT (NP_004173.1; NP_705582.1). -
阳性对照
- Human Kidney and Skeletal Muscle lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab77483 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77483于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Involved in gene transcription regulation. Acts in concert with the corepressor URI1 to regulate androgen receptor transcription (AR). AR N-terminus-associated coactivator which may play a role in facilitating receptor-induced transcriptional activation (PubMed:11854421). Potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling (PubMed:11827465). Increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC (PubMed:17554592). May be a nuclear chaperone that promotes formation of the NF-kappa-B enhanceosome and which is essential for its nuclear function (PubMed:17620405). Suppresses cell transformation and it might mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM (PubMed:17635584). Together with URI1, associates with chromatin to the NKX3-1 promoter region. -
组织特异性
Ubiquitous. Expressed in prostate epithelial cells. Overexpressed in a number of tumor tissues (PubMed:16221885). Highest levels in the heart, skeletal muscle, pancreas, kidney, liver, adrenal gland, peripheral blood leukocytes, lymph node, prostate, and thyroid and the lowest levels in bladder and uterus. -
序列相似性
Belongs to the UXT family. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 465609 Chimpanzee
- Entrez Gene: 525680 Cow
- Entrez Gene: 480900 Dog
- Entrez Gene: 8409 Human
- Omim: 300234 Human
- SwissProt: Q32P97 Cow
- SwissProt: Q9UBK9 Human
- Unigene: 172791 Human
see all -
别名
- Androgen receptor trapped clone 27 protein antibody
- ART 27 antibody
- ART-27 antibody
see all
图片
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Anti-UXT antibody (ab77483) at 0.03 µg/ml + Human Kidney lysate (in RIPA buffer) at 35 µg
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
数据表及文件
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Datasheet download
文献 (1)
ab77483 被引用在 1 文献中.
- Hintermair C et al. Specific threonine-4 phosphorylation and function of RNA polymerase II CTD during M phase progression. Sci Rep 6:27401 (2016). PubMed: 27264542