Anti-USP47抗体(ab72143)
Key features and details
- Rabbit polyclonal to USP47
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-USP47抗体
参阅全部 USP47 一抗 -
描述
兔多克隆抗体to USP47 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to a region between residues 1 and 50 of human USP47 (NP_060414.3)
-
阳性对照
- HeLa whole cell lysate (ab150035).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72143于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
说明 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
-
功能
Putative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues. -
组织特异性
Expressed in skeletal muscle, heart and testis. -
序列相似性
Belongs to the peptidase C19 family. - Information by UniProt
-
数据库链接
- Entrez Gene: 55031 Human
- Entrez Gene: 74996 Mouse
- Omim: 614460 Human
- SwissProt: Q96K76 Human
- SwissProt: Q8BY87 Mouse
- Unigene: 577256 Human
- Unigene: 16974 Mouse
-
别名
- Deubiquitinating enzyme 47 antibody
- TFRP antibody
- Trf TATA binding protein related factor proximal homolog antibody
see all
图片
-
All lanes : Anti-USP47 antibody (ab72143) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 158 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Detection of USP47 by Western Blot of Immunprecipitate.
ab72143 at 0.1µg/ml for WB, using HeLa whole cell lysate immunoprecipitated using ab72143 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab72143 被引用在 5 文献中.
- Yuan F et al. Long non-coding RNA KCNQ1OT1 promotes nasopharyngeal carcinoma cell cisplatin resistance via the miR-454/USP47 axis. Int J Mol Med 47:N/A (2021). PubMed: 33576460
- Hu Y et al. USP47 promotes apoptosis in rat myocardial cells after ischemia/reperfusion injury via NF-κB activation. Biotechnol Appl Biochem 68:841-848 (2021). PubMed: 32761659
- Guo X et al. E2F7 Transcriptionally Inhibits MicroRNA-199b Expression to Promote USP47, Thereby Enhancing Colon Cancer Tumor Stem Cell Activity and Promoting the Occurrence of Colon Cancer. Front Oncol 10:565449 (2020). PubMed: 33489876
- Yan S et al. LINC00668 promotes tumorigenesis and progression through sponging miR-188-5p and regulating USP47 in colorectal cancer. Eur J Pharmacol 858:172464 (2019). PubMed: 31233752
- Sako-Kubota K et al. Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions. Mol Biol Cell 25:3851-60 (2014). PubMed: 25253721