Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (egg extracts)
Specification
egg extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Bands
Specific: 130 kDa Non-specific: 180, 150 kDa
Exposure
10 minute(s) and 0 second(s)
Negative control
HeLa lysates with Usp28 shRNA treatment
Positive control
HeLa lysates with Luc shRNA treatment
Additional data
Additional Notes
One of Usp28 shRNA (#1) did not work well.
Abcam response
We appreciate the valuable data provided in this Abreview. The data shows that our ab110744 performs well when used with human samples however for Xenopus laevis samples no satisfactory results are obtained. As the immunogen of ab110744 shows no identity with the amino acid sequence of Xenopus laevis USP (http://www.uniprot.org/uniprot/Q6DDW3) we would not expect the antibody to detect the target protein in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 02 2014