Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - whole (flight muscles)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
40 µg
Specification
flight muscles
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA, 0.1% TBS Tween
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: HRP anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ecl
Exposure
5 minute(s) and 0 second(s)
Positive control
No observed bands means no observed bands for the drosophila sample. Positive control was mouse skeletal and cardiac tissue the antibody worked great on the positive controls.
Additional data
Additional Notes
Conclusion: The antibody works great for mouse samples, but does not cross-react with the orthologous fly protein
lane 1: Mouse, skeletal muscle
lane 2: Mouse, cardiac muscle
lane 3: Drosophila, skeletal muscle
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback about the lack of cross-reactivity of ab14746 with Drosophila. We appreciate the time and effort that the reviewer has put in to testing this product in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 06 2016