Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA pH8.0
Specification
Brain
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Diluent: TBS
Dilution
1/1500
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Sections were cut and mounted on to glass adhesion slides for staining. The antibody stained specifically, identifying both normal neurons and those exhibiting respiratory deficiency. Staining appeared better in shorter fixed cases (<6 months).
Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit.
SigmaFast DAB tablets were used for the final stage of staining
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Philippa Hepplewhite
Verified customer
提交于 Aug 01 2012