Anti-ULBP2抗体(ab88645)
Key features and details
- Mouse polyclonal to ULBP2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-ULBP2抗体
参阅全部 ULBP2 一抗 -
描述
小鼠多克隆抗体to ULBP2 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human ULBP2 aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9BZM5 -
阳性对照
- Human ovary cancer tissue lysate. Cell lysate from transfected 293T cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88645于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
|
|
Flow Cyt |
Use at an assay dependent concentration.
ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. |
Flow Cyt
Use at an assay dependent concentration. ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
|
靶标
-
功能
Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. -
组织特异性
Expressed in various types of cancer cell lines and in the fetus, but not in normal tissues. -
序列相似性
Belongs to the MHC class I family. -
细胞定位
Cell membrane. Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 80328 Human
- Omim: 605698 Human
- SwissProt: Q9BZM5 Human
- Unigene: 656778 Human
-
别名
- ALCAN alpha antibody
- ALCAN-alpha antibody
- N2DL 2 antibody
see all
图片
-
ab88645 detecting ULBP2 in ULBP2-expressing 293 cells (Green) compared with negative control 293 cells (Black) by flow cytometry.
-
Anti-ULBP2 antibody (ab88645) at 1 µg/ml + Human ovary cancer tissue lysate at 50 µg
Predicted band size: 27 kDa
Observed band size: ~35 kDa why is the actual band size different from the predicted? -
All lanes : Anti-ULBP2 antibody (ab88645) at 1 µg/ml
Lane 1 : cell lysate from ULBP2 transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 27 kDa
Observed band size: ~29 kDa why is the actual band size different from the predicted?
Additional bands at: ~27 kDa, ~33 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
-
Datasheet download
文献 (2)
ab88645 被引用在 2 文献中.
- Marcon F et al. NK cells in pancreatic cancer demonstrate impaired cytotoxicity and a regulatory IL-10 phenotype. Oncoimmunology 9:1845424 (2020). PubMed: 33299656
- Dudek-Peric AM et al. Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin. Cancer Res 75:1603-14 (2015). WB ; Human . PubMed: 25762540