重组Anti-UFM1抗体[EPR4264(2)] (ab109305)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4264(2)] to UFM1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-UFM1抗体[EPR4264(2)]
参阅全部 UFM1 一抗 -
描述
兔单克隆抗体[EPR4264(2)] to UFM1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Fetal kidney, 293T, SH-SY5Y, and HepG2 whole cell lysate (ab7900); Human brain tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4264(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109305于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/1000 - 1/2000. Predicted molecular weight: 9 kDa.
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IP |
1/10 - 1/40.
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IHC-P |
1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 9 kDa. |
IP
1/10 - 1/40. |
IHC-P
1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1. -
序列相似性
Belongs to the UFM1 family. -
细胞定位
Nucleus. Cytoplasm. Predominantly nuclear. Also expressed diffusely in the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 51569 Human
- Entrez Gene: 67890 Mouse
- Entrez Gene: 365797 Rat
- Omim: 610553 Human
- SwissProt: P61960 Human
- SwissProt: P61961 Mouse
- SwissProt: Q5BJP3 Rat
- Unigene: 643655 Human
see all -
别名
- BM 002 antibody
- BM002 antibody
- C13orf20 antibody
see all
图片
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All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/2000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Mouse spleen cell lysate
Lane 4 : Mouse heart cell lysate
Lane 5 : Rat spleen cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 28,9 kDa why is the actual band size different from the predicted?Mouse heart cell lysate Blocking and diluting buffer: 5% NFDM/TBST
The band at around 28kDa might be UFM1 conjugates [PMID: 23152784].
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ab109305 immunoprecipitating UFM1. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.
Lane 1: 293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
Lane 2: 293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109305 in 293 (Human embryonic kidney epithelial cell) whole cell lysate -
ab109305 staining UFM1 in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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ab109305 staining UFM1 in mouse mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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ab109305 staining UFM1 in human thyroid carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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ab109305 staining UFM1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/1000 dilution
Lane 1 : Fetal kidney lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 9 kDa
Observed band size: 9 kDa -
Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109305 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (29)
ab109305 被引用在 29 文献中.
- Zhou J et al. Optimized protocol to detect protein UFMylation in cells and in vitro via immunoblotting. STAR Protoc 3:101074 (2022). PubMed: 35036955
- Zhang J et al. Deficiency of Murine UFM1-Specific E3 Ligase Causes Microcephaly and Inflammation. Mol Neurobiol 59:6363-6372 (2022). PubMed: 35931931
- Wang K et al. Ufl1 deficiency causes skin pigmentation by up-regulation of Endothelin-1. Front Cell Dev Biol 10:961675 (2022). PubMed: 36120581
- Lin M et al. Down-regulated expression of CDK5RAP3 and UFM1 suggests a poor prognosis in gastric cancer patients. Front Oncol 12:927751 (2022). PubMed: 36387125
- Ishimura R et al. The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3. Nat Commun 13:7857 (2022). PubMed: 36543799