Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Sample
Human Cell lysate - other (Heterologously expressed in Lactococcus lactis)
Specification
Heterologously expressed in Lactococcus lactis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 33 kDa Non-specific: 75 kDa
Exposure
10 minute(s) and 0 second(s)
Negative control
Uninduced vector
Additional data
Additional Notes
Non-specific band detected at 75 kDa. Antibody seems to be specific for UCP2 (no bands observed for UCP1, UCP3 or UCP4 using these conditions). Lane 1: marker Lane 2: uninduced vector control Lane 3: UCP1 Lane 4: UCP2 Lane 5: UCP3 Lane 6: UCP4
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Martin King
Verified customer
提交于 Jul 31 2014