Anti-UCP1抗体(ab10983)
Key features and details
- Rabbit polyclonal to UCP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-UCP1抗体
参阅全部 UCP1 一抗 -
描述
兔多克隆抗体to UCP1 -
宿主
Rabbit -
特异性
This polyclonal antibody does not undergo batch testing for mouse western blot, and we cannot therefore guarantee this reagent for this species/application. For an alternative product please see ab209483 which is guaranteed for mouse western blot.
-
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Sheep, Rabbit, Cow, Dog, Human, Macaque monkey -
免疫原
Synthetic peptide corresponding to Human UCP1 aa 145-159 conjugated to keyhole limpet haemocyanin (Cysteine residue).
-
阳性对照
- IHC-P: Murine inguinal white adipose tissue. WB: Rat brown fat lysate.
-
常规说明
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab10983 is affinity purified using the immunogenic peptide immobilized on agarose. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10983于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (11) |
1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples.
|
IHC-P | (8) |
1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue.
|
说明 |
---|
WB
1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples. |
IHC-P
1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue. |
靶标
-
功能
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. -
组织特异性
Brown adipose tissue. -
序列相似性
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 281561 Cow
- Entrez Gene: 7350 Human
- Entrez Gene: 22227 Mouse
- Entrez Gene: 24860 Rat
- Omim: 113730 Human
- SwissProt: P10861 Cow
- SwissProt: P25874 Human
- SwissProt: P12242 Mouse
see all -
形式
UCP1 is preferentially expressed in brown adipose tissue -
别名
- mitochondrial brown fat uncoupling protein antibody
- Mitochondrial brown fat uncoupling protein 1 antibody
- SLC25A7 antibody
see all
图片
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brown adipose tissue staining UCP1 using ab10983 at 1/500 dilution. Detected using an Anti-Rabbit IgG-biotin conjugate and an Avidin-Peroxidase conjugate.
-
ab10983, staining UCP1, in murine inguinal white adipose tissue, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was incubated with primary antibody at 1/1000 dilution and staining was detected using an HRP/DAB detection kit. Sections were counterstained using hematoxylin. -
All lanes : Anti-UCP1 antibody (ab10983) at 1 µg/ml
Lane 1 : Rat brown fat
Lane 2 : Rat brown fat with UCP1 immunizing peptide (human, 145-159)
Secondary
All lanes : Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (722)
ab10983 被引用在 722 文献中.
- Zhu W et al. In-frame deletion of SMC5 related with the phenotype of primordial dwarfism, chromosomal instability and insulin resistance. Clin Transl Med 13:e1007 (2023). PubMed: 36627765
- Hou D et al. Uncoupling protein 1 knockout aggravates isoproterenol-induced acute myocardial ischemia via AMPK/mTOR/PPARα pathways in rats. Transgenic Res 31:107-118 (2022). PubMed: 34709566
- Yang H et al. Effects of maternal undernutrition during late pregnancy on the regulatory factors involved in growth and development in ovine fetal perirenal brown adipose tissue. Anim Biosci 35:1010-1020 (2022). PubMed: 34530507
- Wen J et al. Role of transient receptor potential vanilloid-1 in behavioral thermoregulation of the Mongolian gerbil Meriones unguiculatus. Integr Zool 17:608-618 (2022). PubMed: 34498418
- Abdul Sater Z et al. Combining a β3 adrenergic receptor agonist with alpha-lipoic acid reduces inflammation in male mice with diet-induced obesity. Obesity (Silver Spring) 30:153-164 (2022). PubMed: 34825496