Anti-UBE2I / UBC9抗体(ab21193)
Key features and details
- Goat polyclonal to UBE2I / UBC9
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-UBE2I / UBC9抗体
参阅全部 UBE2I / UBC9 一抗 -
描述
山羊多克隆抗体to UBE2I / UBC9 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Chicken, Hamster, Dog, Xenopus laevis -
免疫原
Synthetic peptide:
SGIALSRLAQERK
, corresponding to N terminal amino acids 2-14 of Human Ube2I. -
常规说明
Product is very sensitive to freeze/thawing therefore this should be avoided. Store at 4C for short term use and -20C for long term storage in small aliquots.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21193于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa).
A 1 hour primary incubation is recommended for this product. Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature. |
说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa). A 1 hour primary incubation is recommended for this product. Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature. |
靶标
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功能
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. -
组织特异性
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. -
通路
Protein modification; protein sumoylation. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. -
细胞定位
Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body. - Information by UniProt
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数据库链接
- Entrez Gene: 374123 Chicken
- Entrez Gene: 491332 Dog
- Entrez Gene: 7329 Human
- Entrez Gene: 25573 Rat
- Entrez Gene: 380450 Xenopus laevis
- Omim: 601661 Human
- SwissProt: P63283 Chicken
- SwissProt: P63279 Human
see all -
别名
- C358B7.1 antibody
- p18 antibody
- SUMO 1 protein ligase antibody
see all
图片
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Anti-UBE2I / UBC9 antibody (ab21193) at 0.3 µg/ml + Rat ovary lysate at 35 µg
Predicted band size: 18 kDa -
Ab21193 (0.3ug/ml) staining human UBE2I in human kidney by immunohistochemistry using paraffin embedded tissue.
Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Anti-UBE2I / UBC9 antibody (ab21193) at 0.1 µg/ml + Human kidney lysate at 35 µg
Predicted band size: 18 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab21193 被引用在 18 文献中.
- Yatskevich S et al. Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation. Cell Rep 34:108929 (2021). PubMed: 33789095
- Guérillon C et al. Multisite SUMOylation restrains DNA polymerase ? interactions with DNA damage sites. J Biol Chem 295:8350-8362 (2020). PubMed: 32350109
- Johnson LM et al. Short-term heritable variation overwhelms 200 generations of mutational variance for metabolic traits in Caenorhabditis elegans. Evolution 74:2451-2464 (2020). PubMed: 32989734
- Xu J et al. A Provocative Molecular Link between Mammographic Density and BRCA1-loss associated TNBC. Int J Hum Genet Genet Disord 1:1-8 (2019). PubMed: 33860286
- Heras G et al. Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification. J Mol Cell Biol 11:356-370 (2019). PubMed: 29868881