重组Anti-UBA3抗体[MILR42872] (ab124728)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MILR42872] to UBA3
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-UBA3抗体[MILR42872]
参阅全部 UBA3 一抗 -
描述
兔单克隆抗体[MILR42872] to UBA3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human UBA3. The exact sequence is proprietary.
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阳性对照
- Lysates of: fetal lung, 293T, HeLa, RAW 264.7, HepG2, K562
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
MILR42872 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124728于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 49 kDa. |
靶标
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功能
Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. -
组织特异性
Ubiquitously expressed. -
通路
Protein modification; protein neddylation. -
序列相似性
Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. - Information by UniProt
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数据库链接
- Entrez Gene: 9039 Human
- Entrez Gene: 22200 Mouse
- Entrez Gene: 117553 Rat
- Omim: 603172 Human
- SwissProt: Q8TBC4 Human
- SwissProt: Q8C878 Mouse
- SwissProt: Q99MI7 Rat
- Unigene: 154320 Human
see all -
别名
- DKFZp566J164 antibody
- EC 6.3.2. antibody
- hUba3 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab124728 被引用在 8 文献中.
- Zhou Q et al. Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells. Nat Commun 13:3034 (2022). PubMed: 35641493
- Zhang S et al. PD-L1 induction via the MEK-JNK-AP1 axis by a neddylation inhibitor promotes cancer-associated immunosuppression. Cell Death Dis 13:844 (2022). PubMed: 36192389
- Yu Q et al. Gossypol inhibits cullin neddylation by targeting SAG-CUL5 and RBX1-CUL1 complexes. Neoplasia 22:179-191 (2020). PubMed: 32145688
- Zhou Q et al. Inhibiting neddylation modification alters mitochondrial morphology and reprograms energy metabolism in cancer cells. JCI Insight 4:N/A (2019). PubMed: 30668548
- Zeng Y et al. An overactive neddylation pathway serves as a therapeutic target and MLN4924 enhances the anticancer activity of cisplatin in pancreatic cancer. Oncol Lett 18:2724-2732 (2019). PubMed: 31404297
- Coleman KE et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). PubMed: 28475037
- Zhu T et al. Neddylation controls basal MKK7 kinase activity in breast cancer cells. Oncogene 35:2624-33 (2016). PubMed: 26364603
- Xu J et al. The neddylation-cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer. Mol Cell Proteomics 14:499-509 (2015). PubMed: 25540389