Anti-Tyrosyl tRNA synthetase/TyrRS抗体[YAR5H08] (ab50961)
Key features and details
- Mouse monoclonal [YAR5H08] to Tyrosyl tRNA synthetase/TyrRS
- Suitable for: WB, IP, ICC
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-Tyrosyl tRNA synthetase/TyrRS抗体[YAR5H08]
参阅全部 Tyrosyl tRNA synthetase/TyrRS 一抗 -
描述
小鼠单克隆抗体[YAR5H08] to Tyrosyl tRNA synthetase/TyrRS -
宿主
Mouse -
经测试应用
适用于: WB, IP, ICCmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Tyrosyl tRNA synthetase/TyrRS.
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阳性对照
- HeLa cells and cell lysate and NIH3T3 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Dibasic monohydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
ab50961 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
克隆
单克隆 -
克隆编号
YAR5H08 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50961于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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IP |
Use at 20 µg/mg of lysate.
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ICC |
1/10.
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说明 |
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WB
1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
IP
Use at 20 µg/mg of lysate. |
ICC
1/10. |
靶标
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功能
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). -
疾病相关
Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. -
序列相似性
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 tRNA-binding domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8565 Human
- Entrez Gene: 107271 Mouse
- Omim: 603623 Human
- SwissProt: P54577 Human
- SwissProt: Q91WQ3 Mouse
- Unigene: 213264 Human
- Unigene: 145488 Mouse
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别名
- CMTDIC antibody
- SYYC_HUMAN antibody
- Tyrosine tRNA ligase, cytoplasmic antibody
see all
图片
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Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 58 kDa -
Immunoprecipititation analysis using 10ug ab50961 per 500ug RIPA HeLa cell lysate. Immunoblot using ab50961 at 1/100 dilution and TrueBlotTM anti-mouse IgG at 1/1000 as secondary antibody.
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Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 58 kDa -
ab50961 at 1/10 dilution staining human YARS in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat anti-mouse IgG at 1/200 as secondary.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab50961 被引用在 2 文献中.
- Jhanji M et al. Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Nat Commun 13:3244 (2022). PubMed: 35688816
- Park SJ et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . PubMed: 26544075