Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain-SNpc)
Specification
Brain-SNpc
Fixative
Formaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% TritonX with 0.1x PBS- 10% Donkeys
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluro 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
IHC/IF image of ab6211 stained human brain sections. The sections were 4% formaldehyde fixed in 0.3% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab ab6211) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. TH expressed in the dopamine positive neurons.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 Apr 13 2012