Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lung w/ B16 cells)
Specification
Lung w/ B16 cells
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Abcam response
Thank you so much for testing this antibody in IHC-Fr.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work in this application. The customer received a discount on a future antibody purchase for testing this product in IHC-Fr. We would welcome additional feedback from other customers as well.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work in this application. The customer received a discount on a future antibody purchase for testing this product in IHC-Fr. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 17 2012