Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
20 µg
Specification
heart
Gel Running Conditions
Reduced Non-Denaturing (Native) (10%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Concentration
1 µg/ml
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TPBS
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/50000
Detection
Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 47 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Xun Ai
Verified customer
提交于 Sep 14 2010