重组Anti-Tryptophan Hydroxylase/TPH抗体[EP1311Y] - BSA and Azide free (ab227845)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1311Y] to Tryptophan Hydroxylase/TPH - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Tryptophan Hydroxylase/TPH抗体[EP1311Y] - BSA and Azide free
参阅全部 Tryptophan Hydroxylase/TPH 一抗 -
描述
兔单克隆抗体[EP1311Y] to Tryptophan Hydroxylase/TPH - BSA and Azide free -
宿主
Rabbit -
特异性
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein
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常规说明
ab227845 is the carrier-free version of ab52954.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1311Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227845于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. |
说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. |
靶标
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组织特异性
Isoform 2 seems to be less widely expressed than isoform 1. -
通路
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. -
序列相似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain. - Information by UniProt
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数据库链接
- Entrez Gene: 7166 Human
- Entrez Gene: 21990 Mouse
- Omim: 191060 Human
- SwissProt: P17752 Human
- SwissProt: P17532 Mouse
- Unigene: 591999 Human
- Unigene: 248684 Mouse
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别名
- Indoleacetic acid 5 hydroxylase antibody
- L tryptophan hydroxylase antibody
- MGC119994 antibody
see all
图片
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This data was developed using ab52954, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Tryptophan Hydroxylase/TPH with Purified ab52954 at 1:2000 dilution (1.191 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. -
Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution + N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
This data was developed using ab52954, the same antibody clone in a different buffer formulation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (9)
ab227845 被引用在 9 文献中.
- Acosta-Andrade C et al. A novel role for antizyme inhibitor 2 as a regulator of serotonin and histamine biosynthesis and content in mouse mast cells. Amino Acids 48:2411-21 (2016). PubMed: 27084713
- Baskar K et al. Functional constituents of a local serotonergic system, intrinsic to the human coronary artery smooth muscle cells. Mol Biol Rep 42:1295-307 (2015). PubMed: 25861735
- Valdés-Fuentes M et al. Effect of daytime-restricted feeding in the daily variations of liver metabolism and blood transport of serotonin in rat. Physiol Rep 3:N/A (2015). WB, IHC . PubMed: 25948822
- Cheng HH et al. Quiescent and proliferative fibroblasts exhibit differential p300 HAT activation through control of 5-methoxytryptophan production. PLoS One 9:e88507 (2014). WB ; Human . PubMed: 24523905
- Dempsie Y et al. Dexfenfluramine and the oestrogen-metabolizing enzyme CYP1B1 in the development of pulmonary arterial hypertension. Cardiovasc Res 99:24-34 (2013). WB ; Human . PubMed: 23519266
- Schallreuter KU et al. Blunted epidermal L-tryptophan metabolism in vitiligo affects immune response and ROS scavenging by Fenton chemistry, part 1: epidermal H2O2/ONOO--mediated stress abrogates tryptophan hydroxylase and dopa decarboxylase activities, leading to low serotonin and melatonin levels. FASEB J 26:2457-70 (2012). ICC/IF ; Human . PubMed: 22415302
- Sauls K et al. Developmental basis for filamin-A-associated myxomatous mitral valve disease. Cardiovasc Res 96:109-19 (2012). Mouse . PubMed: 22843703
- Silva SR et al. The effect of PTEN on serotonin synthesis and secretion from the carcinoid cell line BON. Anticancer Res 31:1153-60 (2011). WB ; Human . PubMed: 21508359
- Mizuno Y et al. Expression of synemin in the mouse spinal cord. Muscle Nerve 39:634-41 (2009). IHC-P ; Mouse . PubMed: 19229966