Application
Western blot
Sample
Human Cell lysate - whole cell (sebocytes)
Loading amount
100 µg
Specification
sebocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% TBST
Other product details
Dilution
1/200
Incubation time
10 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit-POD
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 50, 60, 72, 90 kDa
Positive control
varying
Negative control
varying
Additional data
Additional Notes
I was never sure if the bands were specific because I mostly detected bands below 92 kD. Sometimes the ranges of detection changed even between different clones of one cell line,
Abcam response
The technical support department has investigated this negative review. We have recommended to the reviewer to run a positive control as we know HeLa cells and sebocytes are not known to express high levels of TrkB.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 21 2009