Application
Flow Cytometry
Sample
Rat Cell (PC12 cells (neuronal cells))
Specification
PC12 cells (neuronal cells)
Preparation
Cell harvesting/tissue preparation method: cell culture (commercially available cell line)
Sample buffer: 0,1% Saponin /1% FCS/ PBS
Sample buffer: 0,1% Saponin /1% FCS/ PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0,1% Saponin / PBS
Gating Strategy
no gate
Other product details
Dilution
1/100
Incubation time
45 minute(s) · Temperature: 4°C · Diluent: 0,1% Saponin /1% FCS/ PBS
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit FITC
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/100
Additional data
Additional Notes
neuronal cells:
1 = isotype
2 = untreated cells
3 = treated with rh-ßNGF 50ng/ml
1 = isotype
2 = untreated cells
3 = treated with rh-ßNGF 50ng/ml
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 09 2012