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Triglyceride Quantification Assay Kit (ab65336) provides a sensitive, easy assay to measure triglyceride (TG) concentration in a variety of samples. In the assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a product which reacts with the probe to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
If your sample contains reducing substances such as glucose, fructose or lactose, they are likely to interfere with the assay. In this case, we recommend to use PicoProbe Triglyceride Quantification Assay Kit (Fluorometric) (ab178780).
Visit our FAQs page for tips and troubleshooting.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
This kit also detects monoglycerides and diglycerides.
Some components of this kit are available for purchasing as standalone reagents if you need more quantities (lipase, enzyme mix, TG standard or TG probe). Please contact our team for more details.
|Triglyceride Assay Buffer||1 x 25ml|
|Triglyceride Enzyme Mix (lyophilized)||Green||1 vial|
|Triglyceride Probe (in DMSO, anhydrous)||Red||1 x 200µl|
|Triglyceride Standard (1 mM)||Yellow||1 x 300µl|
Our Abpromise guarantee covers the use of ab65336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Hepatic triglyceride levels was measured using ab65336 in male and female wild-type (WT) or AT2KO (knockout) mice with either normal diet (ND) or high fat diet (HFD).
Fluorometric triglyceride standard curve: mean of duplicates (+/- SD) with background reads subtracted
Triglyceride measured in cell culture lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 40-80 fold and measured fluorometrically.
HepG2 cells were treated with 25 uM Chloroquine for 72h.
Colorimetric triglyceride standard curve using ab65336.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"