重组Anti-Trefoil Factor 3抗体[EPR3974] (ab108599)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3974] to Trefoil Factor 3
- Suitable for: WB, IP, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Trefoil Factor 3抗体[EPR3974]
参阅全部 Trefoil Factor 3 一抗 -
描述
兔单克隆抗体[EPR3974] to Trefoil Factor 3 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human small intestine, colon and uterus tissue lysates. IHC-P: Human colon and colonic adenocarcinoma, breast carcinoma, endometrial carcinoma and papillary carcinoma of thyroid gland tissues. IP: Human small intestine tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 9.60 x 10 -12 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3974 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108599于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
For unpurified use at 1/1000 - 1/10000. |
IP |
1/30.
For unpurified use at 1/10 - 1/100. |
|
IHC-P |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
说明 |
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WB
1/1000. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa). For unpurified use at 1/1000 - 1/10000. |
IP
1/30. For unpurified use at 1/10 - 1/100. |
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
靶标
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功能
Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). -
组织特异性
Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. -
序列相似性
Contains 1 P-type (trefoil) domain. -
细胞定位
Secreted > extracellular space > extracellular matrix > apical lamina. - Information by UniProt
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数据库链接
- Entrez Gene: 7033 Human
- Omim: 600633 Human
- SwissProt: Q07654 Human
- Unigene: 82961 Human
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别名
- hITF antibody
- hP1.B antibody
- Intestinal trefoil factor antibody
see all
图片
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All lanes : Anti-Trefoil Factor 3 antibody [EPR3974] (ab108599) at 1/1000 dilution
Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : TFF3 knockout MCF7 cell lysate
Lane 3 : Human colon cell lysate
Lane 4 : THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Trefoil Factor 3 antibody [EPR3974] staining at 1/1000 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108599 was shown to bind specifically to Trefoil Factor 3. A band was observed at 10 kDa in wild-type MCF7 cell lysates with no signal observed at this size in TFF3 knockout cell line ab274936 (knockout cell lysate ab274994). To generate this image, wild-type and TFF3 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Trefoil Factor 3 with purified ab108599 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-Trefoil Factor 3 antibody [EPR3974] (ab108599) at 1/1000 dilution (purified) + Human colon tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 9 kDa
Observed band size: 9 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-Trefoil Factor 3 antibody [EPR3974] (ab108599) at 1/5000 dilution (purified) + Human small intestine tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 9 kDa
Observed band size: 9 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-Trefoil Factor 3 antibody [EPR3974] (ab108599) at 1/1000 dilution (unpurified)
Lane 1 : Human small intestine tissue lysate
Lane 2 : Human uterus tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 9 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic carcinoma tissue labelling Trefoil Factor 3 with purified ab108599 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Trefoil Factor 3 with unpurified ab108599 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic adenocarcinoma tissue labelling Trefoil Factor 3 with unpurified ab108599 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Trefoil Factor 3 with unpurified ab108599.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial carcinoma tissue labelling Trefoil Factor 3 with unpurified ab108599.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human papillary carcinoma of thyroid gland tissue labelling Trefoil Factor 3 with unpurified ab108599.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human brain tissue labelling Trefoil Factor 3 with unpurified ab108599. Negative staining is shown.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human uterus tissue labelling Trefoil Factor 3 with unpurified ab108599. Negative staining is shown.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muslce tissue labelling Trefoil Factor 3 with unpurified ab108599. Negative staining is shown.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab108599 (purified) at 1/30 immunoprecipitating Trefoil Factor 3 in human small intestine tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab108599 被引用在 21 文献中.
- Bischoff P et al. The single-cell transcriptional landscape of lung carcinoid tumors. Int J Cancer 150:2058-2071 (2022). PubMed: 35262195
- Luo W et al. The expression of trefoil factor 3 is related to histologic subtypes and invasiveness in lung adenocarcinoma. Oncol Lett 21:63 (2021). PubMed: 33281974
- Uhlitz F et al. Mitogen-activated protein kinase activity drives cell trajectories in colorectal cancer. EMBO Mol Med 13:e14123 (2021). PubMed: 34409732
- Xin Y et al. Trefoil Factor 3 Inhibits Thyroid Cancer Cell Progression Related to IL-6/JAK/STAT3 Signaling Pathway. Evid Based Complement Alternat Med 2021:2130229 (2021). PubMed: 34567204
- Fallah S & Beaulieu JF Src family kinases inhibit differentiation of intestinal epithelial cells through the Hippo effector YAP1. Biol Open 10:N/A (2021). PubMed: 34693980