Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Mouse Tissue sections (Kidney, Heart)
Specification
Kidney, Heart
Permeabilization
Yes - 0.1% Tween-20
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA PBS-T
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L), Highl
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Also tested against duodenum sections. Did not observe significant staining, but need to repeat experiment, some possible technical issues - will update review.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 02 2014