Application
Western blot
Sample
Mouse Serum (Mouse serum)
Gel Running Conditions
Non-reduced Denaturing (4-20 % gradient gel)
Loading amount
50 µg
Specification
Mouse serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/15000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Anti rabbit IgG-HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 second(s)
Bands
Specific: approx. 70 kDa
Positive control
Not used
Additional data
Additional Notes
The observed size is just below 70 KDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 11 2017