Application
Western blot
Sample
Hamster Cell lysate - whole cell (BHKT7)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
250000 cells
Specification
BHKT7
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit (Cell Signaling 7074)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
45 second(s)
Bands
Specific: ~100 kDa
Positive control
Lane 1: 293T (human cell line)
Lane 2: BHKT7 (hamster cell line)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 28 2016