Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/4000
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500
Additional data
Additional Notes
Submitted composite image ( ab84036 with arrowheads, another Supplier's antibody lower image).
Upper image shows good, speckled capillary positivity ( pale green arrowheads). However, a subpopulation of astrocytes are strongly positive( pale blue arrowheads).
Fresh brain was snap-frozen.
12 micron sections were fixed in 4% Formalin in PBS for 15 mins at RT.
Sections fixed in acetone gave a similar pattern but not as clear.
Sections fixed in ice-cold ethanol gave no positivity.
Lower image shows no astrocyte positvity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Oct 02 2014