Anti-Transferrin Receptor抗体[B349 (DF1513)] (ab8598)
Key features and details
- Mouse monoclonal [B349 (DF1513)] to Transferrin Receptor
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Transferrin Receptor抗体[B349 (DF1513)]
参阅全部 Transferrin Receptor 一抗 -
描述
小鼠单克隆抗体[B349 (DF1513)] to Transferrin Receptor -
宿主
Mouse -
特异性
This antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond. The specificity of these antibodies, as demonstrated by immunoprecipitation, are equivalent to OKT9, B3/25 and BerT9. This antibody reacts with many proliferating cells in both normal and neoplastic tissues. It also reacts with renal tubular epithelium, islets of Langerhans, scattered cells in the anterior pituitary, hepatocytes and most tissue macrophages. -
经测试应用
适用于: IHC-Fr, ICC/IF, Flow Cyt, WBmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human Transferrin Receptor. Cell line KG1.
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阳性对照
- Tonsil.
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常规说明
This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas. -
克隆
单克隆 -
克隆编号
B349 (DF1513) -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
靶标
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功能
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
疾病相关
Immunodeficiency 46 -
序列相似性
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
翻译后修饰
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
细胞定位
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 7037 Human
- Omim: 190010 Human
- SwissProt: P02786 Human
- Unigene: 529618 Human
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别名
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab8598 被引用在 6 文献中.
- Shytaj IL et al. Alterations of redox and iron metabolism accompany the development of HIV latency. EMBO J 39:e102209 (2020). PubMed: 32157726
- Huang Y et al. TFRC promotes epithelial ovarian cancer cell proliferation and metastasis via up-regulation of AXIN2 expression. Am J Cancer Res 10:131-147 (2020). PubMed: 32064157
- Lehmann M et al. Nanoscale coupling of endocytic pit growth and stability. Sci Adv 5:eaax5775 (2019). PubMed: 31807703
- Perrot G et al. LRP-1--CD44, a new cell surface complex regulating tumor cell adhesion. Mol Cell Biol 32:3293-307 (2012). PubMed: 22711991
- Ortiz-Zapater E et al. Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A. Plant J 48:757-70 (2006). PubMed: 17059402
- Ayala P et al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells. Cell Microbiol 7:1736-48 (2005). PubMed: 16309460