Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with human Torsin A)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
HEK 293 cells transfected with human Torsin A
Blocking step
LI-COR blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Diluent: Odyssey blocking buffer
Concentration
1 µg/ml
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: LI-COR N-IR 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Detection
Detection method
LI-COR Odyssey N-IR scanner
Negative control
Untransfected tissue lysate.
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 35 kDa Non-specific: 60, 110 kDa
Additional data
Additional Notes
Membrane was scanned at default LI-COR Odyssey sensitivity settings. A and B reflects different amounts of human torsin A plasmid that was transfected. Ctl is cells transfected with GFP plasmid, however, the same background bands can be seen there as well (white arrows).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 12 2009