重组Anti-Thyroglobulin抗体[EPR3614] (ab92467)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3614] to Thyroglobulin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Thyroglobulin抗体[EPR3614]
参阅全部 Thyroglobulin 一抗 -
描述
兔单克隆抗体[EPR3614] to Thyroglobulin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Thyroglobulin aa 350-450. The exact sequence is proprietary.
-
阳性对照
- WB: Human thyroid lysate IHC-P: Human papillary thyroid carcinoma
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3614 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92467于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/20000. Predicted molecular weight: 305 kDa.
|
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/10000 - 1/20000. Predicted molecular weight: 305 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). -
组织特异性
Thyroid gland specific. -
疾病相关
Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. -
序列相似性
Belongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains. -
翻译后修饰
Sulfated tyrosines are desulfated during iodination. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 7038 Human
- Omim: 188450 Human
- SwissProt: P01266 Human
- Unigene: 654591 Human
-
别名
- AITD 3 antibody
- AITD3 antibody
- hTG antibody
see all
图片
-
Anti-Thyroglobulin antibody [EPR3614] (ab92467) at 1/10000 dilution + Human thyroid lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 305 kDa -
ab92467, at a 1/2500 dilution, staining Thyroglobulin in paraffin embedded Human papillary thyroid carcinoma tissue by Immunohistochemisty.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab92467 被引用在 3 文献中.
- Wiseman SM et al. Subcutaneous transplantation of human thyroid tissue into a pre-vascularized Cell Pouch™ device in a Mus musculus model: Evidence of viability and function for thyroid transplantation. PLoS One 17:e0262345 (2022). PubMed: 35051203
- Fernando R et al. Expression of thyrotropin receptor, thyroglobulin, sodium-iodide symporter, and thyroperoxidase by fibrocytes depends on AIRE. J Clin Endocrinol Metab 99:E1236-44 (2014). PubMed: 24708100
- Fernando R et al. Human fibrocytes coexpress thyroglobulin and thyrotropin receptor. Proc Natl Acad Sci U S A 109:7427-32 (2012). ICC/IF ; Human . PubMed: 22517745