Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Whole skin)
Specification
Whole skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.1% Tween-20
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/400
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X TBS with serum
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
-Detection was carried out using DAKO ARK (biotin labelling and detection kit)
- We were unable to detect thymine dimers in mouse skin using this antibody.
- We were unable to detect thymine dimers in mouse skin using this antibody.
Abcam response
Thanks for taking the time to submit your review and for your honest feedback. To our knowledge this is the first time ab10347 has been tested for use in IHC-P. It may be that this antibody will not detect its epitope in formalin fixed, paraffin embedded tissue sections. Or perhaps further optimization of the staining protocol is required.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 11 2012