Anti-Thioredoxin / TRX抗体(ab26320)
Key features and details
- Rabbit polyclonal to Thioredoxin / TRX
- Suitable for: IP, WB, ICC/IF
- Reacts with: Human, Chinese hamster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Thioredoxin / TRX抗体
参阅全部 Thioredoxin / TRX 一抗 -
描述
兔多克隆抗体to Thioredoxin / TRX -
宿主
Rabbit -
经测试应用
适用于: IP, WB, ICC/IFmore details -
种属反应性
与反应: Human, Chinese hamster
预测可用于: Mouse, Rat, Sheep, Cow, Cat, Pig -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Thioredoxin/ TRX.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab26320于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use a concentration of 5 µg/ml.
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
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ICC/IF | (2) |
Use at an assay dependent concentration.
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说明 |
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IP
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). -
序列相似性
Belongs to the thioredoxin family.
Contains 1 thioredoxin domain. -
翻译后修饰
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation. -
细胞定位
Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7295 Human
- Entrez Gene: 22166 Mouse
- Entrez Gene: 116484 Rat
- Omim: 187700 Human
- SwissProt: P10599 Human
- SwissProt: P10639 Mouse
- SwissProt: P11232 Rat
- Unigene: 435136 Human
see all -
别名
- ADF antibody
- ATL derived factor antibody
- ATL-derived factor antibody
see all
图片
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Anti-Thioredoxin / TRX antibody (ab26320) at 1 µg/ml +
Recombinant human Thioredoxin / TRX protein (Active) (ab51064) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Exposure time: 30 seconds -
Thioredoxin / TRX was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Thioredoxin / TRX and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab26320.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 12kDa; Thioredoxin / TRX
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Anti-Thioredoxin / TRX antibody (ab26320) at 1 µg/ml + HeLa whole cell lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDaab26320 detects a band of 12 kDa in HeLa, Jurkat and A431 whole cell lysates. The data for the Jurkat and A431 lysates is not shown.
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ab26320, at 1/200 dilution, staining human TRX in assynchronous HeLa cells by ICC/IF.
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Immunofluorescent detection of Thioredoxin /TRX using antibody ab26320. Chinese Hamster Cell (Ovary cells) were fixed in formaldehyde and permeabilized in 0.1% Triton X in PBS/3% BSA. Primary antibody was incubated at 1/1000 for 1h @ 4°C in PBS containing 3% BSA. Secondary antibody: anti-rabbit conjugated to Alexa Fluor® 568 used @ 1/300. CHO cells co-transfected with plasmids encoding GFP and a mutated form of human thioredoxin which does not enter the nucleus, and labelled using ab26320 (1/1000) and Alexa 568 (1/300). Panel [A] demonstrates GFP immunoreactivity. [B ]thioredoxin immunoreactity; many of the same cells are immunoreactive for both GFP and thioredoxin. Panel [D] thioredoxin primary antibody was omitted, hence there is no immunoeactivity.
数据表及文件
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SDS download
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Datasheet download
文献 (39)
ab26320 被引用在 39 文献中.
- Wang L et al. Nitrative inactivation of thioredoxin-1 loses its protective effect in bleomycin-induced pulmonary fibrosis. Int Immunopharmacol 112:109208 (2022). PubMed: 36087509
- Xiong W et al. Low-dose aspirin might alleviate the symptoms of preeclampsia by increasing the expression of antioxidative enzymes. Exp Ther Med 22:1418 (2021). PubMed: 34707700
- Aleksic M et al. The Unity of Redox and Structural Remodeling of Brown Adipose Tissue in Hypothyroidism. Antioxidants (Basel) 10:N/A (2021). PubMed: 33921249
- Rojas Márquez JD et al. Cutting Edge: NOX2 NADPH Oxidase Controls Infection by an Intracellular Bacterial Pathogen through Limiting the Type 1 IFN Response. J Immunol 206:323-328 (2021). PubMed: 33288542
- Sinha D et al. Rapid, direct detection of bacterial topoisomerase 1-DNA adducts by RADAR/ELISA. Anal Biochem 608:113827 (2020). PubMed: 32738213