Application
Western blot
Sample
Mouse Tissue lysate - whole (tail bud e12.5)
Loading amount
5 µg
Specification
tail bud e12.5
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 0.1% milk in TBS
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Detection
Detection method
ecl plus
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Additional data
Additional Notes
a 40kDa band can appear but can wash off with overnight rocking in TBST.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 15 2012