Anti-Tbx3抗体(ab99302)
Key features and details
- Rabbit polyclonal to Tbx3
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Tbx3抗体
参阅全部 Tbx3 一抗 -
描述
兔多克隆抗体to Tbx3 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: ICC/IF, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in nuclear extracts from the following cell lines: HepG2; MCF7. IHC-P (FFPE): Human Placenta (Normal) tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99302于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). |
靶标
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功能
Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. -
组织特异性
Widely expressed. -
疾病相关
Defects in TBX3 are the cause of ulnar-mammary syndrome (UMS) [MIM:181450]. UMS is characterized by ulnar ray defects, obesity, hypogenitalism, delayed puberty, hypoplasia of nipples and apocrine glands. -
序列相似性
Contains 1 T-box DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 374202 Chicken
- Entrez Gene: 6926 Human
- Entrez Gene: 21386 Mouse
- Entrez Gene: 353305 Rat
- Omim: 601621 Human
- SwissProt: O15119 Human
- SwissProt: P70324 Mouse
- SwissProt: Q7TST9 Rat
see all -
别名
- Bladder cancer related protein XHL antibody
- T box 3 antibody
- T-box protein 3 antibody
see all
图片
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ab99302 staining Tbx3 in HepG2 cells. The cells were fixed with 4% Paraformaldehyde (10min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab99302 at 5μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) pre-adsorbed, at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) pre-adsorbed, at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Perkin Elmer, Operetta CLS).
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All lanes : Anti-Tbx3 antibody (ab99302) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 2 :MCF7 nuclear extract lysate (ab14860)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Additional bands at: 22 kDa, 77 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Ab99302 should recognise all three isoforms of Tbx3. We are probably observing Isoform I and Isoform II on this Western Blot image. The molecular weights of Isoform I and Isoform II are 77- and 79-kDa respectively. -
ab99302 staining Tbx3 in the AR42J-B13 rat pancreatic tumor cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton/PBS and blocked with blocking buffer for 60 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/5000.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab99302 被引用在 26 文献中.
- Tsujikawa K et al. Developmentally interdependent stretcher-compressor relationship between the embryonic brain and the surrounding scalp in the preosteogenic head. Dev Dyn 251:1107-1122 (2022). PubMed: 34997665
- Ishibashi R et al. Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci Rep 12:17775 (2022). PubMed: 36272994
- Li H et al. Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing. Cells 12:N/A (2022). PubMed: 36611882
- Damerell V et al. The c-Myc/TBX3 Axis Promotes Cellular Transformation of Sarcoma-Initiating Cells. Front Oncol 11:801691 (2021). PubMed: 35145908
- Peres J et al. TBX3 Promotes Melanoma Migration by Transcriptional Activation of ID1, which Prevents Activation of E-Cadherin by MITF. J Invest Dermatol 141:2250-2260.e2 (2021). PubMed: 33744299