Anti-Tbx2抗体(ab33298)
Key features and details
- Rabbit polyclonal to Tbx2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Tbx2抗体
参阅全部 Tbx2 一抗 -
描述
兔多克隆抗体to Tbx2 -
宿主
Rabbit -
特异性
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Dog, Xenopus laevis -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Tbx2.
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阳性对照
- This antibody gave a positive signal in the following Human tissue lysates: Kidney; Lung; Heart
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33298于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. -
组织特异性
Expressed primarily in adult in kidney, lung, and placenta. Weak expression in heart and ovary. -
序列相似性
Contains 1 T-box DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 448779 Dog
- Entrez Gene: 6909 Human
- Entrez Gene: 21385 Mouse
- Omim: 600747 Human
- SwissProt: Q863A2 Dog
- SwissProt: Q13207 Human
- SwissProt: Q60707 Mouse
- Unigene: 531085 Human
see all -
别名
- FLJ10169 antibody
- HGNC:11597 antibody
- T box 2 antibody
see all
图片
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Formalin-fixed paraffin-embedded mouse bone marrow section stained with ab33298 Tbx2 antibody at 1/500 dilution.
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All lanes : Anti-Tbx2 antibody (ab33298) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 74 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab33298 被引用在 5 文献中.
- Durbin AD et al. Selective gene dependencies in MYCN-amplified neuroblastoma include the core transcriptional regulatory circuitry. Nat Genet 50:1240-1246 (2018). PubMed: 30127528
- Cho GS et al. Tbx2 regulates anterior neural specification by repressing FGF signaling pathway. Dev Biol 421:183-193 (2017). PubMed: 27913219
- Lv Y et al. TBX2 over-expression promotes nasopharyngeal cancer cell proliferation and invasion. Oncotarget 8:52699-52707 (2017). PubMed: 28881763
- Liu F et al. TBX2 expression is regulated by PAX3 in the melanocyte lineage. Pigment Cell Melanoma Res 26:67-77 (2013). PubMed: 23020925
- Lüdtke TH et al. Tbx2 controls lung growth by direct repression of the cell cycle inhibitor genes Cdkn1a and Cdkn1b. PLoS Genet 9:e1003189 (2013). PubMed: 23341776