Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole fish)
Loading amount
10 µg
Specification
whole fish
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: DaKo Goat Anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Positive control
Transgenic zebrafish expressing human Tau
Additional data
Additional Notes
The antibody clearly recognizes human Tau expressed in zebrafish but It also gives many unspecific bands that could be endogenous zebrafish Tau according to the size (50-75 KDa aprox), but there are others that seem unspecific so it is difficult to know.
Abcam response
Thank you for participating in Abtrial testing. The antibody is guaranteed in WB using human samples. Zebrafish on this occasion did not work, unfortunately.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 27 2015