Anti-TYRO3抗体[OTI5B7] (ab117877)
Key features and details
- Mouse monoclonal [OTI5B7] to TYRO3
- Suitable for: Flow Cyt (Intra), IP, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-TYRO3抗体[OTI5B7]
参阅全部 TYRO3 一抗 -
描述
小鼠单克隆抗体[OTI5B7] to TYRO3 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), IP, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human TYRO3. Protein expressed in HEK-293T cells.
Database link: Q06418 -
阳性对照
- ICC/IF: pCMV6-ENTRY TYRO3 cDNA transfected COS-7 cells. Flow Cyt (Intra): HEK-293T cells transfected with overexpress plasmid. IP: DDK tagged TYRO3 overexpression lysates.
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常规说明
The clone number has been updated from 5B7 to OTI5B7, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI5B7 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117877于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
IP |
Use a concentration of 4 µg/ml.
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ICC/IF |
1/100.
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说明 |
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Flow Cyt (Intra)
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IP
Use a concentration of 4 µg/ml. |
ICC/IF
1/100. |
靶标
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功能
May be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor. -
组织特异性
Abundant in the brain and lower levels in other tissues. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7301 Human
- Omim: 600341 Human
- SwissProt: Q06418 Human
- Unigene: 381282 Human
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别名
- Brt antibody
- BYK antibody
- DTK antibody
see all
图片
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pCMV6-ENTRY TYRO3 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for TYRO3 using ab117877 at a 1/100 dilution in ICC/IF.
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For each experiment, 500 µl of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK-293T lysate), 2 µg of ab117877 and 20 µl (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Lane 1 : Negative control: IP without adding ab117877
Lane 2 : Anti-TYRO3 antibody [OTI5B7] (ab117877) at 2 µg
All lanes : -
HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY TYRO3 (Red) or empty vector control plasmid (Blue) were immunostained with ab117877 at 1/100 dilution and then analyzed by Flow Cytometry (Intracellular).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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