Application
Western blot
Sample
Salmo salar Cell lysate - whole cell (cell lines transfected with GFP-TYK2)
Loading amount
100000 cells
Specification
cell lines transfected with GFP-TYK2
Gel Running Conditions
Reduced Denaturing (4 - 12 %)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS +/- BLOCKING REAGENT
Secondary antibody
Name
Non-Abcam antibody was used: goat sc-2004 from santa cruz biotechnology
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 100, 80, 65, 50, 30 kDa
Positive control
CELLS TRANSFECTED WITH GFP-TYK2 AND BLOTTED WITH aGFP FROM abcam
Negative control
not transfected cells
Additional data
Additional Notes
many bands were observed but non related to the expected size based on the size detected by aGFP.
Abcam response
It seems that this antibody either does not work in Western blot or does not crossreact with the Tyk2 protein of Salmo salar. We encourage all researchers which have experience with this antibody in Western blot or with other species to provide also their feedback. Thank you.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 03 2012