Application
Western blot
Sample
Human Cell lysate - whole cell (EA hy926)
Specification
EA hy926
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 30 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: BD Biosciences Anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal West Pico
Exposure
10 second(s)
Bands
Specific: 55 kDa
Positive control
None
Negative control
No selenite added
Additional data
Additional Notes
Regarding the Westen Blot image:
Lane #1 (leftmost lane): 1:1 dilution of whole cell lysate (with solubilization mix)
Lane #2: 1:2 dilution of whole cell lysate (with solubilization mix)
Lane #3: 1:4 dilution of whole cell lysate (with solubilization mix)
Thus as you can see by the image, dilutions of the lysate result in reduced expression of Thioredoxin reductase
Lane #1 (leftmost lane): 1:1 dilution of whole cell lysate (with solubilization mix)
Lane #2: 1:2 dilution of whole cell lysate (with solubilization mix)
Lane #3: 1:4 dilution of whole cell lysate (with solubilization mix)
Thus as you can see by the image, dilutions of the lysate result in reduced expression of Thioredoxin reductase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Adam Szadkowski
Verified customer
提交于 Nov 10 2005