Anti-TSLP抗体(ab47943)
Key features and details
- Rabbit polyclonal to TSLP
- Suitable for: IHC-P, ICC/IF, WB, ELISA, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TSLP抗体
参阅全部 TSLP 一抗 -
描述
兔多克隆抗体to TSLP -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WB, ELISA, Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length TSLP protein (Human),>98% pure
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 100ul of sterile water to 1mg/ml. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was purified by affinity chromatography employing immobilized hTSLP matrix. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47943于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 18 kDa). Detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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ELISA |
Use at an assay dependent concentration.
This allows the detection of 0.2 - 0.4 ng/well of recombinant human TSLP, using 100µl/well antibody solution. |
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Sandwich ELISA |
Use at an assay dependent concentration.
To detect hTSLP by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab84247 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP. |
说明 |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 18 kDa). Detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ELISA
Use at an assay dependent concentration. This allows the detection of 0.2 - 0.4 ng/well of recombinant human TSLP, using 100µl/well antibody solution. |
Sandwich ELISA
Use at an assay dependent concentration. To detect hTSLP by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab84247 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP. |
靶标
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功能
Cytokine that induces the release of T cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. -
组织特异性
Expressed in a number of tissues including heart, liver and prostate. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 85480 Human
- Omim: 607003 Human
- SwissProt: Q969D9 Human
- Unigene: 389874 Human
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别名
- Thymic stromal lymphopoietin antibody
- Thymic stromal lymphopoietin protein TSLP antibody
- Tslp antibody
see all
图片
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ab47943 detecting TSLP in sandwich ELISA
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Anti-TSLP antibody (ab47943) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa, 51 kDa, 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
The 28 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to TSLP.
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab47943 被引用在 28 文献中.
- Numazaki M et al. ASP7266, a Novel Antibody against Human Thymic Stromal Lymphopoietin Receptor for the Treatment of Allergic Diseases. J Pharmacol Exp Ther 380:26-33 (2022). PubMed: 34728559
- Jung SY et al. Wnt-activating human skin organoid model of atopic dermatitis induced by Staphylococcus aureus and its protective effects by Cutibacterium acnes. iScience 25:105150 (2022). PubMed: 36193049
- Hu YQ & Zhang JZ A Comparison for Type 2 Cytokines and Lesional Inflammatory Infiltrations in Bullous Pemphigoid and Atopic Dermatitis. Clin Cosmet Investig Dermatol 15:2313-2321 (2022). PubMed: 36325102
- Jia J et al. An Amide Alkaloid Isolated from Ephedra sinica Ameliorates OVA-Induced Allergic Asthma by Inhibiting Mast Cell Activation and Dendritic Cell Maturation. Int J Mol Sci 23:N/A (2022). PubMed: 36362328
- Chan LKY et al. Short-Form Thymic Stromal Lymphopoietin (sfTSLP) Is the Predominant Isoform Expressed by Gynaecologic Cancers and Promotes Tumour Growth. Cancers (Basel) 13:N/A (2021). PubMed: 33652749