Anti-TRPM8抗体(ab85617)
Key features and details
- Rabbit polyclonal to TRPM8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRPM8抗体
参阅全部 TRPM8 一抗 -
描述
兔多克隆抗体to TRPM8 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Dog, Monkey, Baboon -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in the following human whole cell lysates: HeLa; PC3.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85617于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa). |
靶标
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功能
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. -
组织特异性
Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors. -
序列相似性
Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 79054 Human
- Entrez Gene: 171382 Mouse
- Omim: 606678 Human
- SwissProt: Q7Z2W7 Human
- SwissProt: Q8R4D5 Mouse
- Unigene: 366053 Human
- Unigene: 218753 Mouse
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别名
- Long transient receptor potential channel 6 antibody
- LTrpC-6 antibody
- LTrpC6 antibody
see all
图片
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All lanes : Anti-TRPM8 antibody (ab85617) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab85617 被引用在 2 文献中.
- Amato A et al. TRPM8 Channel Activation Reduces the Spontaneous Contractions in Human Distal Colon. Int J Mol Sci 21:N/A (2020). PubMed: 32751347
- Bidaux G et al. Regulation of Activity of Transient Receptor Potential Melastatin 8 (TRPM8) Channel by Its Short Isoforms. J Biol Chem 287:2948-62 (2012). WB ; Human . PubMed: 22128173