重组Anti-TRPM7 + TRPM6抗体[EPR4582] - BSA and Azide free (ab232455)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4582] to TRPM7 + TRPM6 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TRPM7 + TRPM6抗体[EPR4582] - BSA and Azide free -
描述
兔单克隆抗体[EPR4582] to TRPM7 + TRPM6 - BSA and Azide free -
宿主
Rabbit -
特异性
The immunogen used for this product shares 86% homology with TRPM6 (ten amino acid stretch with 100% homology). ab109438 (The same clone in a diffrent buffer) has been shown to cross react with TRPM6 in WB.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: JAR whole cell lysate.
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常规说明
ab232455 is the carrier-free version of ab109438.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4582 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232455于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 213 kDa.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 213 kDa. |
靶标
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细胞定位
TRPM7: Membrane. TRPM6: Multi-pass membrane protein. -
数据库链接
- Entrez Gene: 140803 Human
- Entrez Gene: 54822 Human
- Omim: 605692 Human
- Omim: 607009 Human
- SwissProt: Q5VYG5 Human
- SwissProt: Q96LV9 Human
- SwissProt: Q96QT4 Human
- SwissProt: Q9BX84 Human
see all
图片
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Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/1000 dilution + JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 213 kDaBlocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109438).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (4)
ab232455 被引用在 4 文献中.
- Guo S et al. LncRNA HOTAIR sponges miR-301a-3p to promote glioblastoma proliferation and invasion through upregulating FOSL1. Cell Signal 94:110306 (2022). PubMed: 35292358
- Xia Q et al. Inhibition of SENP6 restrains cerebral ischemia-reperfusion injury by regulating Annexin-A1 nuclear translocation-associated neuronal apoptosis. Theranostics 11:7450-7470 (2021). PubMed: 34158860
- Wan J et al. TRPM7 Induces Tumorigenesis and Stemness Through Notch Activation in Glioma. Front Pharmacol 11:590723 (2020). PubMed: 33381038
- Wan J et al. TRPM7 Induces Mechanistic Target of Rap1b Through the Downregulation of miR-28-5p in Glioma Proliferation and Invasion. Front Oncol 9:1413 (2019). PubMed: 31921670