Application
Western blot
Sample
Rat Tissue lysate - other (artery)
Loading amount
50 µg
Specification
artery
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: ANTI-RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~ 100 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 07 2010