Anti-TRIM32抗体(ab96612)
Key features and details
- Rabbit polyclonal to TRIM32
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRIM32抗体
参阅全部 TRIM32 一抗 -
描述
兔多克隆抗体to TRIM32 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a sequence within amino acids 1-259 of Human TRIM32 (AAH03154).
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阳性对照
- 293T and MOLT4 whole cell lysates; H441 Xenograft.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96612于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 72 kDa.
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IHC-P |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/500.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 72 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/500. |
靶标
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功能
Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo. -
组织特异性
Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Limb-girdle muscular dystrophy 2H (LGMD2H) [MIM:254110]: An autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
Bardet-Biedl syndrome 11 (BBS11) [MIM:209900]: A syndrome characterized by usually severe pigmentary retinopathy, early-onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Bardet-Biedl syndrome inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for clinical manifestation of some forms of the disease. Note=The disease is caused by mutations affecting the gene represented in this entry. -
序列相似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 5 NHL repeats.
Contains 1 RING-type zinc finger. -
翻译后修饰
Ubiquitinated. -
细胞定位
Cytoplasm. Localized in cytoplasmic bodies, often located around the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 521975 Cow
- Entrez Gene: 22954 Human
- Entrez Gene: 69807 Mouse
- Entrez Gene: 313264 Rat
- Omim: 602290 Human
- SwissProt: Q13049 Human
- SwissProt: Q8CH72 Mouse
- Unigene: 591910 Human
see all -
别名
- 72 kda Tat interacting Protein antibody
- 72 kDa Tat-interacting protein antibody
- BBS11 antibody
see all
图片
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Anti-TRIM32 antibody (ab96612) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 72 kDa
7.5% SDS PAGE. -
ab96612 at 1/100 dilution staining TRIM32 in H441 Xenograft by Immunohistochemistry, Paraffin-embedded tissue.
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Immunofluorescence analysis of paraformaldehyde-fixed A431, using TRIM32(ab96612) antibody at 1:500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab96612 被引用在 7 文献中.
- Zhao L et al. mTORC1-c-Myc pathway rewires methionine metabolism for HCC progression through suppressing SIRT4 mediated ADP ribosylation of MAT2A. Cell Biosci 12:183 (2022). PubMed: 36371321
- Luo Q et al. TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma. Cell Rep 30:98-111.e5 (2020). PubMed: 31914402
- Lazzari E et al. Analysis of the Zn-Binding Domains of TRIM32, the E3 Ubiquitin Ligase Mutated in Limb Girdle Muscular Dystrophy 2H. Cells 8:N/A (2019). PubMed: 30884854
- Wilczynska A et al. eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5'UTR. Genome Biol 20:262 (2019). PubMed: 31791371
- Ito M et al. Overexpression of E3 ubiquitin ligase tripartite motif 32 correlates with a poor prognosis in patients with gastric cancer. Oncol Lett 13:3131-3138 (2017). PubMed: 28521418
- Ruelas DS et al. MicroRNA-155 Reinforces HIV Latency. J Biol Chem 290:13736-48 (2015). PubMed: 25873391
- Crawford LJ et al. Synergistic effects of proteasome inhibitor carfilzomib in combination with tyrosine kinase inhibitors in imatinib-sensitive and -resistant chronic myeloid leukemia models. Oncogenesis 3:e90 (2014). WB ; Human . PubMed: 24590311