Anti-RNF216抗体(ab25961)
Key features and details
- Rabbit polyclonal to RNF216
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-RNF216抗体
参阅全部 RNF216 一抗 -
描述
兔多克隆抗体to RNF216 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Mouse RNF216 (N terminal).
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阳性对照
- Mouse heart cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25961于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).
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说明 |
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IHC-P
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa). |
靶标
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功能
Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3 inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis. -
组织特异性
Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Gordon Holmes syndrome -
序列相似性
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers. -
结构域
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. -
翻译后修饰
Auto-ubiquitinated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 108086 Mouse
- SwissProt: P58283 Mouse
- Unigene: 381440 Mouse
- Unigene: 488397 Mouse
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别名
- CAHH antibody
- E3 ubiquitin ligase TRIAD3 antibody
- E3 ubiquitin-protein ligase RNF216 antibody
see all
图片
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Lane 1 : Anti-RNF216 antibody (ab25961) at 0.5 µg/ml
Lane 2 : Anti-RNF216 antibody (ab25961) at 1 µg/ml
Lane 3 : Anti-RNF216 antibody (ab25961) at 2 µg/ml
All lanes : Mouse heart cell lysates
Predicted band size: 99 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
ab25961 at 10µg/ml staining RNF216 in mouse heart by IHC
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Immunofluorescence of RNF216 in Mouse Heart cells using ab25961 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab25961 被引用在 4 文献中.
- Evankovich J et al. Toll-Like Receptor 8 Stability is Regulated by Ring Finger 216 in response to circulating MicroRNAs. Am J Respir Cell Mol Biol N/A:N/A (2019). PubMed: 31385713
- Rajagopalan S et al. TNFR-associated factor 6 and TGF-ß-activated kinase 1 control signals for a senescence response by an endosomal NK cell receptor. J Immunol 192:714-21 (2014). PubMed: 24337384
- Xu C et al. Regulation of autophagy by E3 ubiquitin ligase RNF216 through BECN1 ubiquitination. Autophagy 10:2239-50 (2014). PubMed: 25484083
- Miah SM et al. Ubiquitylation of an internalized killer cell Ig-like receptor by Triad3A disrupts sustained NF-?B signaling. J Immunol 186:2959-69 (2011). PubMed: 21270397