Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293T-TRF1 HEK293T-TRF2)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
HEK293T-TRF1 HEK293T-TRF2
Blocking step
(agent) for 1 hour(s) and 10 minute(s) · Concentration: 2% · Temperature: 37°C
Other product details
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 63kDA kDa
Additional data
Additional Notes
IHC and WB of tranfected HEK293T-TRF1 and HEK293T-TRF2 show that TRF1 (TRF-78) cross react with TRF2 protein
This information has to be specified in the ab datasheet. If not the use of this antibody will led to the publication and perpetuation of erroneous research results.
Abcam response
Thank you for your valuable feedback. We are sorry to see there are some concerns regarding the results. Mouse species has not been tested before with this antibody.
The reviewer has used this antibody and found a cross-reactivity with the TRF2 protein. The issue was investigated with the customer and by our quality control team. So far, we are not concerned about the quality and specificity of this antibody for TRF1. The homology of TRF1 and TRF2 is 23% which is not likely to lead to cross reactivity. The sample type was dedicated as mouse. Since it is a overexpression experiment in HEK293T cells we assume that the plasmids used were derived from the murine sequence. Please note, that this product is not covered by our Abpromise for this species.
The customer was offered a Credit note in compensation.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Giovanna Roncador
Verified customer
提交于 Jul 24 2015