Application
Western blot
Loading amount
7.5 µg
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Sample
Zebrafish Tissue lysate - whole (embryos 5-7 days post fertilization)
Specification
embryos 5-7 days post fertilization
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase labelled anti-rabbit antibody
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 55-58 kDa
Exposure
35 minute(s) and 0 second(s)
Positive control
58 Kd using Jurkat cells
Additional data
Additional Notes
Despite not clear band was observed with the expected side (61kd), the bands we visualize sometimes could be correct. Because of this we are still fine tunning the WB conditions.
Abcam response
Thank you for your review of this untested species. Please feel free to update us if your results improve.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Rub�n Valued Customer
Verified customer
提交于 Oct 22 2013