Application
Western blot
Sample
Human Cell lysate - whole cell (LnCaP cells)
Loading amount
50 µg
Specification
LnCaP cells
Gel Running Conditions
Reduced Denaturing (5-15% gradient gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
supersignal pico chemiluminescent substr
Exposure
5 second(s)
Bands
Specific: 55 kDa
Positive control
LnCaP cells with or without TRAF4 overexpression
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 14 2012