Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
A region within synthetic peptide: ADVSIVYDQQ SRRSRGFAFV YFENVDDAKE AKERANGMEL DGRRIRVDFS, corresponding to internal sequence amino acids 145-194 of Human TRA2B
293T Cell Lysate (for WB applications) and Skeletal muscle cells (for Immunohistochemistry)
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver.
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain.
Phosphorylated in the RS domains.
Dimethylation at Arg-241 is probably asymmetric.
Information by UniProt
arginine/serine-rich 10 antibody
Arginine/serine-rich splicing factor 10 antibody
Western blot - TRA2B antibody (ab50846)
Anti-TRA2B antibody (ab50846) at 5 µg/ml (in 5% skim milk / PBS buffer) + 239T Cell Lysate at 10 µg
HRP conjugated anti-Rabbit IgG should be diluted in 1/ 50,000 - 100,000
Predicted band size:
Observed band size:
36 kDa (
why is the actual band size different from the predicted?
Gel Concentration: 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2B antibody (ab50846)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling TRA2B with ab50846 at 4-8µg/ml. Arrows indicate positively stained human skeletal muscle cells. Magnification: 400X.
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