Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse ES cells)
Specification
mouse ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Amersham ECL Anti-rabbit IgG, HRP-Linked Species-S
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
luminata Forte Western HRP Substrate
Bands
Specific: 33 kDa Non-specific: 27, 85 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
293T human cells
Additional data
Additional Notes
Figure Legend:
MW marker: 175, 80, 58, 46, 30, 23kD
Lanes 1, 3, 5: 293T cells
Lanes 2, 4, 6: mouse ES cells (129Sv)
Antibody dilutions:
Lanes 1-2: 1/250
Lanes 3-4: 1/500
Lanes 5-6: 1/1000
Additional exposures available upon request
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 11 2013