Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (Spindles in X. laevis cytoplasmic egg extract)
Specification
Spindles in X. laevis cytoplasmic egg extract
Fixative
Formaldehyde and Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 3% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Thank you for your valuable feedback. Xenopus was not a tested species for ab32795, and it seems unclear whether this antibody does recognize TPX2 under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 17 2011